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Proteintech arpc4 polyclonal antibody
Arp2/3 complex participated in the oocyte differentiation process. A) The Arp2 protein expression levels during oocyte differentiation process. B,C) <t>Arpc4</t> and Arp2 knockdown were achieved by using Arpc4 siRNA ( n = 24 in each group). D) Arpc4 and F‐actin fluorescence intensity significantly decreased by using Arpc4 siRNA. E) Oocyte morphology and volume in NC‐siRNA and Arpc4‐siRNA group, scale bar = 50 µm. F) The DDX4 staining of oocytes in NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. G) The polarity degree of oocytes in NC‐siRNA and Arpc4‐siRNA group. H) The rate of oocytes with double nuclei in NC‐siRNA and Arpc4‐siRNA group. I) The proportion of oocyte with a ring Golgi apparatus in NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. J) The relative ATP5a fluorescence intensity in oocytes of NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. K) The relative calreticulin fluorescence intensity in oocytes of NC‐siRNA and Arpc4‐siRNA group, scale bar = 10 µm. L) The Arpc4 and Arp2 protein expression levels in NC‐siRNA, Arpc4‐siRNA and Rescue group. M,N) Arpc4 and F‐actin fluorescence intensity in NC‐siRNA, Arpc4‐siRNA and Rescue group. O) The rate of double nuclei, polarity degree and volume of oocytes in NC‐siRNA, Arpc4‐siRNA, and the Rescue group. P) The proportion of oocyte with a ring Golgi apparatus in NC‐siRNA, Arpc4‐siRNA, and the Rescue group, scale bar = 20 µm. Q) The relative ATP5a fluorescence intensity in oocytes of NC‐siRNA, Arpc4‐siRNA, and the Rescue group, scale bar = 20 µm. * meant p < 0.05, ** meant p < 0.01, *** meant p < 0.001, and **** meant p < 0.0001.
Arpc4 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Arp2/3 complex participated in the oocyte differentiation process. A) The Arp2 protein expression levels during oocyte differentiation process. B,C) Arpc4 and Arp2 knockdown were achieved by using Arpc4 siRNA ( n = 24 in each group). D) Arpc4 and F‐actin fluorescence intensity significantly decreased by using Arpc4 siRNA. E) Oocyte morphology and volume in NC‐siRNA and Arpc4‐siRNA group, scale bar = 50 µm. F) The DDX4 staining of oocytes in NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. G) The polarity degree of oocytes in NC‐siRNA and Arpc4‐siRNA group. H) The rate of oocytes with double nuclei in NC‐siRNA and Arpc4‐siRNA group. I) The proportion of oocyte with a ring Golgi apparatus in NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. J) The relative ATP5a fluorescence intensity in oocytes of NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. K) The relative calreticulin fluorescence intensity in oocytes of NC‐siRNA and Arpc4‐siRNA group, scale bar = 10 µm. L) The Arpc4 and Arp2 protein expression levels in NC‐siRNA, Arpc4‐siRNA and Rescue group. M,N) Arpc4 and F‐actin fluorescence intensity in NC‐siRNA, Arpc4‐siRNA and Rescue group. O) The rate of double nuclei, polarity degree and volume of oocytes in NC‐siRNA, Arpc4‐siRNA, and the Rescue group. P) The proportion of oocyte with a ring Golgi apparatus in NC‐siRNA, Arpc4‐siRNA, and the Rescue group, scale bar = 20 µm. Q) The relative ATP5a fluorescence intensity in oocytes of NC‐siRNA, Arpc4‐siRNA, and the Rescue group, scale bar = 20 µm. * meant p < 0.05, ** meant p < 0.01, *** meant p < 0.001, and **** meant p < 0.0001.

Journal: Advanced Science

Article Title: Microfilament‐Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice

doi: 10.1002/advs.202500358

Figure Lengend Snippet: Arp2/3 complex participated in the oocyte differentiation process. A) The Arp2 protein expression levels during oocyte differentiation process. B,C) Arpc4 and Arp2 knockdown were achieved by using Arpc4 siRNA ( n = 24 in each group). D) Arpc4 and F‐actin fluorescence intensity significantly decreased by using Arpc4 siRNA. E) Oocyte morphology and volume in NC‐siRNA and Arpc4‐siRNA group, scale bar = 50 µm. F) The DDX4 staining of oocytes in NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. G) The polarity degree of oocytes in NC‐siRNA and Arpc4‐siRNA group. H) The rate of oocytes with double nuclei in NC‐siRNA and Arpc4‐siRNA group. I) The proportion of oocyte with a ring Golgi apparatus in NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. J) The relative ATP5a fluorescence intensity in oocytes of NC‐siRNA and Arpc4‐siRNA group, scale bar = 20 µm. K) The relative calreticulin fluorescence intensity in oocytes of NC‐siRNA and Arpc4‐siRNA group, scale bar = 10 µm. L) The Arpc4 and Arp2 protein expression levels in NC‐siRNA, Arpc4‐siRNA and Rescue group. M,N) Arpc4 and F‐actin fluorescence intensity in NC‐siRNA, Arpc4‐siRNA and Rescue group. O) The rate of double nuclei, polarity degree and volume of oocytes in NC‐siRNA, Arpc4‐siRNA, and the Rescue group. P) The proportion of oocyte with a ring Golgi apparatus in NC‐siRNA, Arpc4‐siRNA, and the Rescue group, scale bar = 20 µm. Q) The relative ATP5a fluorescence intensity in oocytes of NC‐siRNA, Arpc4‐siRNA, and the Rescue group, scale bar = 20 µm. * meant p < 0.05, ** meant p < 0.01, *** meant p < 0.001, and **** meant p < 0.0001.

Article Snippet: MYH9 Polyclonal antibody (11128‐1‐AP), MYH10‐Specific Polyclonal antibody (19673‐1‐AP), MYH14 Polyclonal antibody (20716‐1‐AP), MYO7A Polyclonal antibody (20720‐1‐AP), TEX14 Polyclonal antibody (18351‐1‐AP), ARP2 Polyclonal antibody (10922‐1‐AP), ARPC4 Polyclonal antibody (10930‐1‐AP), ROCK1 Polyclonal antibody (21850‐1‐AP), ROCK2 Monoclonal antibody (66633‐1‐Ig) and RHOA Polyclonal antibody (10749‐1‐AP) were purchased from Proteintech (Wuhan, China).

Techniques: Expressing, Knockdown, Fluorescence, Staining